The BD LSRFortessa™ X-20 cell analyzer delivers high performance multicolor analysis with the most compact footprint in its class. The BD LSRFortessa X-20 can be configured with up to 5 lasers, which enables the detection of up to 20 parameters simultaneously. The most common laser choices are blue, red, violet, yellow-green, and UV.

This dye offers improved brightness over Pacific Orange™ and reduced spillover into the FITC channel when compared to AmCyan. BD Horizon V500 cannot be

Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b. BD LSR Fortessa SORP – How to Use Guide 2 Section 1: Instrument Startup 1 The instrument is: OFF (go to step 2); ON (go to step 4). 2 Turn ON the cytometer in the power extension switch. Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first.

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Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b. bdbiosciences.com 23-11093-00 Rev. A 3/2010 For Research Use Only Becton, Dickinson and Company BD Biosciences San Jose, CA 95131 Tel 877.232.8995 Fax 800.325.9637 Complete Optical Configuration of the Fortessa1 Our second Fortessa has exactly the same configuration as our first fortessa on the violet, yellow and red laser paths, but a different configuration on the blue laser and the addition of 2 channels on Ultra-Violet 355nm Optical Configuration of the Fortessa2 (our only UV laser instrument) Faculty of Medicine Flow Cytometry Facility - BD Fortessa X20 (special order system) 4- laser Configuration Blue (488 nm) Red (640 nm) Violet (405 nm) Yellow/Green BD LSRFORTESSA -20 5 Emission spectra Excitation laser line (nm) Fluorescence channel Fluorochromes provided by BD Biosciences 488 Green FITC Alexa Fluor® 488 Yellow PE PI Orange BD Horizon™ PE-CF594 PE-Texas Red® Red 7-AAD PE-Cy™5 PerCP PerCP-Cy™5.5 Infrared PE-Cy™7 532b or 561b Yellow PE PI Orange BD Horizon PE-CF594 PE-Texas Red® Red PE-Cy5 BD Accuri™ C6 BD FACSCalibur™ BD FACSVerse™* BD FACSCanto™ II BD LSRFortessa™ X-20 BD FACSAria™ Product Family BD Influx™ BD FACSJazz™ Excitation Laser Line Filter Relative Brightness Violet Laser (405 nm) •••••• 405 nm 450/40 BD Horizon™ Brilliant Violet™ 421 (BV421) (Ex max 407 nm/Em max BD FACS LSRFortessa HTS Operation Manual 1 FACS LSRFortessa Standard Operation Protocol HTS Mode You are strongly recommended to adjust voltage for each channel and setup compensation with Tube Mode before plate mode acquisition! (Please refer to LSR Fortessa Standard Operation Protocol – Basic operation for Tube Mode) The Fortessa has a 405nm, 488nm, 561nm and 633nm laser setup with the ability to detect signals in 16 channels. Before designing an experiment or purchasing an antibody, please ensure it is compatible with this instrument.

Laser. Excitation laser. Fluorescence channel.

contains a bandpass filter and a dichroic mirror for that channel. The filter holder has an ID chip that identifies the holder to the system so the software can confirm

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Choose what results to return. Education; Research; Library; For students; For employees; About UiB. About UiB; Strategy

2 Turn ON the cytometer in the power extension switch.

Flow cytometry studies were performed using a LSRII Fortessa (Becton streptavidin) were obtained from BD Biosciences, eBioscience, or Biolegend 30 .
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--- Empty Waste&n BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler). BD LSRFortessa X 20. The facility at 2117A Everitt Lab (click link for Due to its emission profile, BV480 has less spillover into the BV605, BV650 and BV711 channels and, in general, is brighter than BV510. Due to its excitation BD Biosciences delivers software and workstations that are intended for running the instruments given channel, the first listed fluorophore is added by default. For cell sorting, the FCRC at RU is equipped with three BD FACSAria Cell Sorters has five lasers (355, 405, 488, 561 and 640 nm) and 64 fluorescence channels.

Remove the sheath container lid. 5. BD LSR Fortessa (16 colour Analyser) 4 lasers, 16 filters (see table below). Sample loading via tubes.
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Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm).

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The BD LSRFortessa™ X-20 cell analyzer delivers high performance multicolor analysis with the most compact footprint in its class. The BD LSRFortessa X-20 can be configured with up to 5 lasers, which enables the detection of up to 20 parameters simultaneously. The most common laser choices are blue, red, violet, yellow-green, and UV.

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